Regulatory

Part:BBa_K1444012

Designed by: Dan Ziemianowicz   Group: iGEM14_Calgary   (2014-10-08)

Composite promoter and consensus B. subtilis RBS - pTetR x2

A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by two copies of the repressible promoter pTetR BBa_R0040, and ending with the consensus RBS from B. subtilis BBa_K090505.

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pTetR repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The duplicated repressible promoter allows for stronger repression.

IMPORTANT NOTE: Our experience with this entire series of repressible promoters suggests that they are unstable in the standard biobrick vector pSB1C3. As such, they have been submitted to the registry in a pUC57 vector BBa_K1444027.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None